Abstract: Optimization of the solid substrate and culture conditions was a very important measure to decrease production cost,increase enzyme activity and realize industrial production of cellulase. In this experiment, solid fermentation of TrichodermaKoningii F244 in line with uniform design U15(58) had been performed with two different temperatures culture, as 30℃ duringthe first 30 h and at 27℃ in the following hours continuously. Consequently, the activities of FPA, CMCase, cotton lyase andβ-glucosidase were regarded as dependant in Multivariate Regression Analysis in SPSS and then the mathematical model of overallcellulase activity was constructed by adding the corresponding activity equations together with weight. Furthermore, themaximum of cellulase model was figured out by constraint mathematical programming. It revealed that in cellulase production bysolid fermentation with two temperatures culture from Trichoderma Koningii, 72h were proper fermentation time under theconditions of natural aeration, natural substrate pH(about 6.5) and an environmental humidity of 60%. Furthermore, ideal solidsubstrate constituents were rice straw powder 50%, wheat bran 42%, (NH4)2SO4 8% and water about 4.9 time weight of solidconstituents. Moreover, Tween80 in small quantity was helpful for enzyme activity.

Key words: Trichoderma Koningii, solid fermentation, FPA, CMCase, cotton lyase, β-glucosidase