FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (9): 152-157.

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Determination of Nonvolatile Organic Acid, Fatty Acid and Low Molecular Sugars in Tomato by Capillary Gas Chromatography

 ZHU  Xiao-Lan, GAO  Yun, SU  Qing-De   

  1. Tobacco Research Center, Chemistry Department, University of Science and Technology of China
  • Online:2004-09-15 Published:2011-10-24

Abstract: Two capillary gas chromatography methods for the determination of low molecular sugars and nonvolatile organicacid in tomato are described. After crush, tomato samples were extracted from water under ultrasonic surge, then decompressed,desiccated and derivatized with BSTFA+1%TMCS, and analyzed by capillary gas cbromatography. As to determination oforganic acid of tomato, tomato samples was reacted with H2SO4-CH3OH (volume ratio 10%) for two hours, and extracted threetimes with CH2Cl2, then analyzed by capillary gas cbromatography. The optimization strategies for sample preparation and CGCwere discussed in this work. The result suggested that the main low molecular sugars in tomato were glucose and fructose, andthe main acid were citric acid, malic acid and palmitic acid. Moreover, this method has been used to analyze sample of differenttype of tomatoes such as ordinary tomatoes and cherry tomatoes with a satisfactory resolution reproducibility.

Key words: capillary gas chromatography, low molecular sugra, tomato, nonvolatile organic aci, derivation