Activity Assay on β-galactosidase Produced by L.acidophilus In-1 and L.acidophilus Lakcid

Abstract

Abstract: The activity of β-galactosidase produced by both L.acidophilus Ind-1 and L.acidophilus Lakcid was studied byusing MRS as culture medium. The results showed that the extraction rate of β-galactosidase was higher using 1%SDS:chloroform=1:10 to break the cell wall; that 2.229μ moleONP/L.min and 1.808μmoleONP/L.min β-galactosidase were formedby the two stains after 36h culture at 37℃;and that the math models of this enzyme accumulated in MRS were Logistic andMistcherlich.

Key words: Lactobacillus acidophilus, β-galactosidase, math model

ZHAO Rui-Xiang, SUN Jun-Liang, YU Tao. Activity Assay on β-galactosidase Produced by L.acidophilus In-1 and L.acidophilus Lakcid[J]. FOOD SCIENCE, 2003, 24(12): 52-54.

[1]	HUANG Junyi, WANG Fengna, WU Xiang, LIU Shan, LI Cong, LI Xinfu, XU Baocai. Effect of Mixed Starter Cultures on the Quality of Fermented Sausage [J]. FOOD SCIENCE, 2020, 41(24): 95-101.
[2]	ZHANG Qiuyue, WANG Gang, CHEN Kunpeng, PAN Daodong, ZENG Xiaoqun, WU Zhen, GUO Yuxing. Effect of Lactobacillus acidophilus on Regulating the Production of Immune Factors in HT-29 Cells under Initial Weak Acidic and Alkaline Conditions [J]. FOOD SCIENCE, 2020, 41(19): 191-197.
[3]	ZHANG Nana, LIU Yang, YU Yi, ZHAO Yu, DOU Tonghai, XU Qiong, DUAN Wenfeng, WENG Shiyu. Real-Time Fluorescent Quantitative PCR Method for Detection of Lactobacillus acidophilus in Probiotic Beverages [J]. FOOD SCIENCE, 2019, 40(8): 27-32.
[4]	WU Jing, WANG Wenwen, XIONG Ronglu, WU Zhen, PAN Daodong, ZENG Xiaoqun, GUO Yuxing. Cloning, Expression and Characterization of Sortase A from Lactobacillus acidophilus ATCC4356 [J]. FOOD SCIENCE, 2018, 39(4): 76-81.
[5]	WANG Yong, JIA Yan, REN Xiaodong, MING Zhu, JIANG Yan, ZHAO Pei, PANG Guangchang, YAN Yali, CHEN Qingsen. Extracellular Proteins from Lactobacillus acidophilus Regulate the Activation of Critical Proteins Involved in the MAPK and PI3K-AKT Signaling Pathways [J]. FOOD SCIENCE, 2017, 38(3): 155-163.
[6]	JIA Yan, LI Maomao, CHEN Rui, JIANG Yan, MING Zhu, ZHAO Pei, PANG Guangchang, YAN Yali, CHEN Qingsen. Effect of Eucommia ulmoides Oliv. Bark Aqueous Extract on the Growth and Fermentation Products of Lactobacillus acidophilus [J]. FOOD SCIENCE, 2016, 37(23): 147-153.
[7]	1.河南科技学院食品学院，河南新乡 453003；2.河南科技学院生命科技学院，河南新乡 453003. Antibacterial Mechanism of Lactobacillin XH2 from Lactobacillus acidophilus on Escherichia coli [J]. FOOD SCIENCE, 2015, 36(3): 75-79.
[8]	HAO Fengqi, LI Jingmei, YANG Guilian. Influence of Lactobacillus acidophilus on Intestinal Mucosal Immunity in a Mouse Model of Stress Induced by Auto-Activity Restriction [J]. FOOD SCIENCE, 2015, 36(23): 247-252.
[9]	XIE Fang, PAN Hanxu,YUAN Shuzhi, WANG Jiao, HOU Qingying, CAO Jiankang*. Effects of Infiltration with Yeast Mannan on Postharvest Storage of Tomato Fruit [J]. FOOD SCIENCE, 2015, 36(2): 221-225.
[10]	WU Zhen1, PAN Daodong1,2,*, GUO Yuxing1, ZENG Xiaoqun2, SUN Yangying2. Immunoregulatory Activity of Lactobacillus acidophilus Peptidoglycan in Mouse Intestinal Mucosa [J]. FOOD SCIENCE, 2015, 36(13): 207-210.
[11]	ZHAO Rui-xiang, LI Gang, NIU Sheng-yang, YANG Da-guang, LU Si-hai, DUAN Gai-li, LI Yin-na. Purification and Enzymatic Properties of Bile Salt Hydrolase Produced by Lactobacillus acidophilus [J]. FOOD SCIENCE, 2014, 35(5): 165-168.
[12]	YANG Xiang-yi1，MAN Chao-xin2，LIU Ying1，WANG Jin-yu1，LANG You1，DONG Xin-yue1，YAN Tian-wen1，JIANG Yu-jun1, 2,*. Regulation of Exopolysaccharide Produced by Lactobacillus acidophilus NCFM on Immune-Related Genes in Mice [J]. FOOD SCIENCE, 2013, 34(5): 213-217.
[13]	YE Yu-dan，CHAI Chun-yan，LIU Guo-yan，WANG Yi-ru. Development of Photoelectric Sensor for Detection of E. coli [J]. FOOD SCIENCE, 2013, 34(18): 185-188.
[14]	YANG Tian-you1，DUAN Gai-li2，ZHAO Rui-xiang2,*，NIU Sheng-yang2，YUAN Zheng2. Biological Characteristics of Lactobacillin XH1 Bacteriocin Produced by Lactobacillus acidophilus [J]. FOOD SCIENCE, 2013, 34(17): 197-200.
[15]	GE Wu-peng1，WANG Xiu-ling1，ZHAO Rui1，CHEN Ying2，YANG Jing3，QIN Li-hu4. Comparison of Fermentation Properties of Lactobacillus acidophilus in Cow and Goat Milk [J]. FOOD SCIENCE, 2013, 34(15): 186-189.

Activity Assay on β-galactosidase Produced by L.acidophilus In-1 and L.acidophilus Lakcid

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments