Abstract: Objective: To observe the regulation of germanium-enriched barley seedling (GBSE)on the immune function of mice by detecting the cellular immunity and humoral immunity. Methods: After given different dose of GEBS by gavage,a series of items were detected on mice,including phagocytosis test of macrophage on abdominal cavity,quantity and classification of white blood cell (WBC),titer of antibody,quantity of antibody forming cell and weights of immune organ. Results: Three doses of GEBS all can obviously increase phagocytic function of macrophages (p<0.01) and increase the quantity of WBC (p< 0.05); The medium dose and high dose increase the titer of anti-CRBC antibody (p<0.01) and the quantity of antibody-forming cell (p<0.01) obviously; The low dose and medium dose increase spleen index (p<0.01) and the low dose group increase liver index (p<0.05). Conclusion: GEBS can improve cellular immunity and humoral immunity of mice obviously,which is positively related to the dose of GEBS; GEBS can also increase the weight of immune organ,and there is certain correlationship between the weight gain and the dose of EGBS.

Key words: GEBS, immune function, mice