Abstract: In this study,the royal jelly samples were dissolved in phosphorus solution(pH 2) containing protein precipitator to extract streptomycin residue by means of ultrasonic.The sample extract was cleaned up by cation exchange column and reverse-phase column,while the streptomycin was eluted off via SPE cartridge with methanol and evaporate to dryness on nitrogen dry evaporator,and the residues were dissolved in mobile phase and then passed through 0.45 μm filter for determination by a liquid chromatography FLD detection with post-column derivatization.A Atlantis HILIC Silica(2.1 mm×150 mm,3.0 μm) was as chromatographic separation column,and the mixed solution of acidic aqueous solution and acetonitrile was as mobile phase under gradient elution.The total analysis time is less than 25 min.The spiked recoveries are 71.7% to 77.4%.The limit of detection of the method is 10 μg/kg.

Key words: royal jelly, streptomycin, HPLC-FLD, post-column derivatization