FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 193-196.

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Speedy Method of Making Template DNA in Analysis of Tea Germplasm Based on AFLP Fingerprint

 ZHOU  Li-Hua, ZHAO  Chao-Yi, LUO  Jun-Wu, HUANG  Jian-An, TAN  He-Ping   

  1. 1.Department of Tea Science, College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China; 2.Key Laboratory of Tea Science, Ministry of Education, Changsha 410128, China; 3.Tea Research Institute, Guangdong Academy of Agricultural Sciences, Yingde 513042, China; 4.Institute of National Measurment and Testing Technology, Chengdu 610021, China
  • Online:2007-02-15 Published:2011-12-31

Abstract: Genomic DNA was isolated once from tea plant by chloroform and isoamyl alcohol. The results showed that the values of OD260/OD280 for DNA samples obtained by this method are between 1.70 and 1.85 respectively with the molecular weight of DNA higher than 23kb. The DNAs have been completely digested by EcoR I. Amplified figures in the PCR, and AFLP fingerprinting figures are all obtained.

Key words: tea plant, AFLP(amplified fragment length polymorphism), DNA extraction