Study on DNA Extraction from Fresh Fruit for Detection of Genetically Modified Organism

doi:10.7506/spkx1002-6630-200903052

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3): 231-234.doi: 10.7506/spkx1002-6630-200903052

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Study on DNA Extraction from Fresh Fruit for Detection of Genetically Modified Organism

DUAN Jing-jing1，XIAO Lin1,*，CHEN Jun2

(1.State Key Laboratory of Pollution Control and Resource Reuses, School of Environment, Nanjing University, Nanjing 210093,
China ；2.Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou 215004, China)

Received:2008-03-07 Revised:2008-08-02 Online:2009-02-01 Published:2010-12-29
Contact: DUAN Jing-jing1 E-mail:duanjingjing_23@163.com

Abstract

Abstract:

This study optimized the method of modified CTAB from fruit fleshes such as papaya, ect. The results showed that the optimum amount of lyophilized fruit powder is 0.10 g and the best proportion of polyvinyl pyrrolidone (PVP) added into extraction buffer is 1.0%. Furthermore, by this method, the effects of polyphenolics and polysaccharides in fleshes are effectively removed, the concentration of isolated genomic DNAs is high, and the value of A260/A280 is between 1.80 and 2.00. Compared to SDS method and DNA extraction kit, this modified CTAB method is better. Using DNAs isolated from papaya, cantaloupe, banana and pear as templates, endogenous rbcL genes are amplified through PCR reaction and objective bands of about 433 bp are obtained.

Key words: genetically modified organism (GMO), fruits, DNA extraction

CLC Number:

DUAN Jing-jing1，XIAO Lin1,*，CHEN Jun2. Study on DNA Extraction from Fresh Fruit for Detection of Genetically Modified Organism[J]. FOOD SCIENCE, 2009, 30(3): 231-234.

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Study on DNA Extraction from Fresh Fruit for Detection of Genetically Modified Organism

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