Pectinase-Extraction with PEG/（NH4）2 SO4 Two-Phase Aqueous System

Abstract

Abstract: The extraction behavior of pectinase in two-phase aqueous system was investigated. The effects of the molecular weight and concentration of polyethylene glycol, pH, the concentrations of NaCl and extract temperature on the extraction coefficient K were studied. The results showed that the optimum conditions are as follows: the mass composition in two-phase aqueous system PEG1000 27%, (NH4)2SO4 19%, NaCl 0.002%, pH5.0, the highest extraction coefficient K obtained in experiments can reach 14.

Key words: two-phase aqueous extraction, pectinase, partition coefficient

YANG Hui, ZHANG Juan, WANG Xu. Pectinase-Extraction with PEG/（NH4）2 SO4 Two-Phase Aqueous System[J]. FOOD SCIENCE, 2007, 28(6): 124-127.

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Pectinase-Extraction with PEG/（NH4）2 SO4 Two-Phase Aqueous System

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