Screening of Two Bacterial Strains Capable of Degrading Citrus Pomace and Their Characteristics

doi:10.7506/spkx1002-6630-201423037

Abstract

Abstract:

This study was conducted to screen microorganisms degrading citrus pomace. Five strains of citrus pomacedegrading
bacteria were screened from soil compost and tested for citrus essential oil toleranc. Two of these strains
were found to have higher tolerance. Meanwhile, cellulase and pectinase activities of the two selected strains were also
determined, and their ability to degrade citrus pomace was characterized. The experimental results showed that the selected
strains L207 and L702 could grow normally at 65 ℃ as thermophilic stains. They could tolerate up to 25.0% aqueous
ethanol and produce 1.31 and 2.08 times higher cellulase activity, and 12.68 and 7.30 times higher pectinase activity than the
control Bacillus subtilis L520, respectively. Both strains were able to rapidly and efficiently degrade cellulose and pectin.
16S rDNA sequencing showed that they both belonged to Bacillus licheniformis.

Key words: citrus pomace degrading bacteria, citrus essential oil, cellulase, pectinase, 16S rDNA

CLC Number:

Q939.96

LI Shi-zhong1, HUANG Jian-guo1, LI Zhi-ling 1, PENG Liang-zhi2, LI Yong 1,*. Screening of Two Bacterial Strains Capable of Degrading Citrus Pomace and Their Characteristics[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201423037.

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Screening of Two Bacterial Strains Capable of Degrading Citrus Pomace and Their Characteristics

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