Flask Fermentation study on Carotenoid Composition of the Hyper Producing Strain Rhodotorrula.sp

Abstract

Abstract: The carotenoid composition,courses of fermentation and optimum fermentation conditions were studied in carotenoid hyperproducing strain Rhodotoru sp,whose principal carotenoid pigment was β-carotenone.The pigments were accumulated at the end of exponential phase and the stationary phase.The cell biomass,carotenoid content and yield of strain Rhodotoru sp could reach 8.72g/L、1359.7μg/g and 11.86mg/L respectively under the selected optimum conditions. The optimum fermentation conditions for carotenoid production were as follows:sucrose 30g/L,(NH4)2SO410g/L,yeast extract 3g/L,MgCl20.3g/L,medium 100ml(in 500ml flask),steady pH6.0,inoculum size 30ml/L and culture time 72h at 28℃ and 150r/min.

Key words: Carotenoid , β-carotenone , HPLC , Fermentation condition

CANG Yi-Hua, LU Ling, WANG Wei, WANG Gang, LIANG Ming-Xiang. Flask Fermentation study on Carotenoid Composition of the Hyper Producing Strain Rhodotorrula.sp[J]. FOOD SCIENCE, 2002, 23(5): 34-38.

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Flask Fermentation study on Carotenoid Composition of the Hyper Producing Strain Rhodotorrula.sp

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