Isolation of β-Glucosidase-Producing Endophytes from Glycyrrhiza inflate and Their Effects on Flavonoid Transformation

Abstract

Abstract: Forty-seven endophytes were isolated from different parts (root, stem and leaf) of Glycyrrhiza inflate and potent 2 β-glucosidase-producing strains, designated as GF10 and GF19, were screened out of them. GF10 and GF19 were comparatively evaluated for their effectiveness in microbial transformation of flavonoid glucosides in Glycyrrhiza inflate into aglycones to obtain higher antioxidant activity. Both strains could increase the antioxidant activity of flavonoids, and the DPPH radical scavenging rates after fermentation with them were 71.67% and 65.94%, respectively, which were 246.57% and 218.86% higher than before fermentation (20.68%). HPLC analysis showed that the concentrations of liquiritin and isoliquiritin decreased to different extents after microbial transformation, whereas the concentrations of liquiritigenin and isoliquiritigenin significantly increased. Liquiritigenin was the most abundant among four flavonoids with a percentage of 29.18% after GF19 transformation, which was 181.12% higher than the original level (10.38%), while GF10 transformation made isoliquiritigenin the most dominant flavonoid and its percentage was increased from its original level (4.27%) to 8.64% by 102.34%.

Key words: licorice, endophytes, β-glucosidase, licorice flavonoids, transformation

CLC Number:

References

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