Determination of Enrofloxacin in Soy Bean Sprout by LC-MS/MS

Abstract

Abstract: This paper reports a sensitive and specific LC-MS/MS method for the determination of enrofloxacin in soy bean sprout. Samples were extracted with 0.1 mol/L NaOH, cleaned up on a Waters Oasis MAX anion exchange column, and separated by RP HPLC using a mobile phase consisting of acetonitrile and 0.1% formic acid for gradient elution. Enrofloxacin was quantitatively and qualitatively analyzed by ESI-MS/MS in the positive ion scan MRM mode. The method showed a linear range of 1–100 μg/kg and a limit of detection of 0.3 μg/kg. The average spike recovery rates of enrofloxacin in blank sample was 91.6%–94.0% with RSD of 6.6%.

Key words: high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), enrofloxacin, difloxacin, soy bean sprout

CLC Number:

References

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