Abstract:

In order to generate a monoclonal antibody with specificity to simultaneously detect enrofloxacin and ciprofloxacin, glutaraldehyde was used to prepare the artificial antigens with common structures of enrofloxacin and ciprofloxacin exposed as the major epitopes. Balb/c mice were immunized with the prepared immunogens. Results indicated that glycosylated BSA used for immunogen synthesis resulted in a higher antiserum titer when compared to unglycosylated BSA. A monoclonal antibody with respective IC50 values of 9.6 ng/mL and 10.2 ng/mL to enrofloxacin and ciprofloxacin was successfully prepared through cell fusion.

Key words: enrofloxacin, ciprofloxacin, glycosylated carrier, veterinary drug residues, artificial antigen, immunological effect