FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (6): 139-142.doi: 10.7506/spkx1002-6630-201306030

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Multiplex PCR for detection of Transgenic Rice with Bar and Bt gene

QIU Liang-yan1,XIAO You-yu2,LIU Jia1,SONG Shang-xin1,XIAO Hong-mei1,*,SUO Na1,WANG Yu-jian1,WANG Xue-qiang1   

  1. 1. Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture, College of Food Science and Technology, Nanjing AgriculturalUniversity, Nanjing 210095, China;2. Nanjing Institute of Supervision & Testing on Product Quality, Nanjing 210028, China
  • Received:2011-11-25 Revised:2013-01-28 Online:2013-03-25 Published:2013-03-01

Abstract: Based on the DNA sequences of rice endogenous SPS gene and exogenous Bar, Bt, CaMV35S promoter, Ubiquitin promoter and NOS terminator genes, specific primers were selected to establish a multiplex polymerase chain reaction (PCR) detection system for transgenic rice with Bar and Bt genes. This multiplex PCR system could allow accurate and rapid detection of six target genes, and the sensitivity was 0.9%.

Key words: transgenic rice, detection, multiplex PCR

CLC Number: