Determination of Lutein and Zeaxanthin in Pangasius bocouti by Reversed-Phase High Performance Liquid Chromatography

Abstract

Abstract: A high performance liquid chromatography method was established for the determination of 1utein and zeaxanthin in Pangasius bocouti. The chromatographic separation was achieved on a C30 column using a mobile phase made up of acetonitrile, methanol and spasmolytol (75:25:0.05, V/V) by gradient elution. The detection wavelength was set at 450 nm, and an external standard method was used to quantify 1utein and zeaxanthin. An excellent linear relationship of this method for 1utein and zeaxanthin was observed (R2 ＞0.999). The average spike recovery rates of 1utein and zeaxanthin were 85.43%－96.60% and 83.18%－96.18% with relative standard deviations of 5.01%－7.15% and 4.60%－8.43%. The limit of detection was 0.008 mg/kg for 1utein and 0.003 mg/kg for zeaxanthin. This method was sensitive, repeatable, and suitable for the determination of 1utein and zeaxanthin aquatic products.

Key words: reversed-phase high performance liquid chromatography, lutein, zeaxanthin, Pangasius bocouti

CLC Number:

TS207.3

References

[1] 冷向军,李小勤. 水产动物着色的研究进展[J]．水产学报, 2006, 30(1): 138-143.
[2] CHEW B P, PARK J S. Carotenoid action on the immune response[J]. J Nutr, 2004, 134(1): 257S-261S.
[3] KRINSKY N I, LANDRUM J T, BONE R A. Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye[J]. Annu Rev Nutr, 2003, 23: 171-201.
[4] KRINSKY N I. Possible biologic mechanisms for a protective role of xanthophylls[J]. J Nutr, 2002, 132(3): 540S-542S.
[5] MARES-PERLMAN J A, MILLEN A E, FICEK T L, et a1. The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic diseases[J]. J Nutr, 2002, 132 (3): 518S-524S.
[6] TABUNOKI H, SUGIYAMA H, TANAKA Y, et a1. Isolation, characterization, and cDNA sequence of a carotenoid binding protein from the silk gland of Bombyx mori larvae[J]. J Biol Chem, 2002, 277(35): 32133-32140.
[7] CHEN H M, MEYERS S P , HARDY R W, et a1. Color stability of astaxanthin pigmented rainbow trout under various packaging conditions[J]. J Food Sci, 1984, 49: 1337-1340.
[8] FOSS P, STOREBAKKEN T, SCHIEDT K, et a1. Carotenoids in diets for Salmonids. I. Pigmentation of rainbow trout with the individual optical isomers of astaxanthin in comparison with canthaxanthin[J]. Aquaculture, 1984, 41: 213-226.
[9] TORRISSEN O J, NAEVDAL G. Pigmentation of salmonids-genetical variation in carotentoid deposition in rainbow trout. Aquaculture[J], 1984, 38: 59-66.
[10] CHRISTOPHERSEN A G, KNUTHSEN P, SKIBSTED L H. Determination of carotentoids in Salmonids. Z Lebensm Unters Forsch[J], 1989, 188: 413-418.
[11] TORRISSEN O J. Pigmentation of salmonids- a comparison of astaxanthin and canthaxanthin as pigment sources for rainbow trout[J]. Aquaculture, 1986, 53: 271-278.
[12] SIGURGISLADOTTIR S, PARRISH C C, LALL S P, et a1. Effects of feeding natural tocopherols and astaxanthin on Atlantic salmon (Salmo salar) fillet quality[J]. Food Res Int, 1994, 27: 23-32.
[13] ANDERSEN H J, BERTELSEN G, CHRISTOPHERSEN A G, et a1. Development of rancidity in salmonoid steaks during retail display. A comparison ofpractical storage life of wild salmon and farmed rainbow trout[J]. Lebensmittel-Untersuchung und-Forschung, 1990. 191: 119-122.

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