FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (20): 65-68.

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Optimization of Alcoholysis Reaction for Two-Step Enzymatic Synthesis of  MLM-Type Structural Lipid

  

  • Received:2011-07-28 Revised:2012-08-24 Online:2012-10-25 Published:2012-11-09

Abstract: In order to obtain monoglyceride (MAG) with high purity for the synthesis of MLM-type structure lipid, immobilized lipase TL IM from Thermomyces lanuginose was used to catalyze the synthesis of MAG by alcoholysis of rapeseed oil and anhydrous ethanol. The optimal alcoholysis conditions were determined by one-factor-at-a-time and orthogonal array design as follows: rapeseed oil/anhydrous ethanol molar ratio of 1:5, lipase dosage of 7% (m/m, on the basis of the weight of substrates), reaction temperature of 35 ℃ and reaction time of 18 h, resulting in an MAG content of 38.82% in the final products. The purity of the MAG obtained could be increased to 90.76% by a three-stage molecular distillation process.

Key words: MLM structure lipid, rapeseed oil, anhydrous ethanol, alcoholysis, monoglycerides

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