Optimization of Extraction Process for Flavonoids from Chrysanthemum nankingense by Response Surface Methodology

Abstract

Abstract: Box-Behnken experimental design and response surface analysis were used to optimize the extraction of flavonoids from Chrysanthemum nankingense. The results showed that the optimal process conditions for the extraction of flavonoids from Chrysanthemum nankingense were 78% ethanol solution as the solvent at a material-to-liquid ratio of 1:41, extraction time of 89 min, and extraction temperature of 68℃. Under these conditions, the yield of flavonoids was 2.88 mg/g.

Key words: Chrysanthemum nankingense, flavonoids, response surface methodology, extraction

CLC Number:

Q946.83

References

[1] 汤庚国,李湘萍. 江苏野菜资源的利用与开发[J].植物资源与环境,1995,4(3):35-36.
[2] 肖玫,刘彪,李林.马齿苋、菊花脑的矿物质元素测定及其开发应用[J]. 粮食加工与食品机械,200,6: 32-33.
[3] 姜秀娟,唐金成,白红进,等.可见分光光度法测定刺山柑花蕾中总黄酮含量[J].食品科学, 2010,31(18): 59-63.
[4] 魏永生,王永宁,石玉平,等.分光光度法测定总黄酮含量的实验条件研究[J].青海大学学报:自然科学版,2003,21(3):61-63.
[5] 尉芹,王冬梅,马希汉,等.杜仲叶总黄酮的测定[J].西北农林科技大学学报, 2001,29(5):119-123.
[6] Mei-Hua Lee, Chuan-Chuan Lin. Comparison of techniques for extraction of isoflavones from the root of Radix Pereira: Ultrasonic and pressurized solvent extractions[J]. Food Chem, 2007,105:223-228．
[7]Yan-ling Wang, Guang-Sheng Xi, Yong-Chun，et al. Microwave-assisted extraction of flavonoids from Chinese herb Radix puerariae (Ge Gen)[J]. Journal of Medicinal Plants Research, 2010, 4(4):304-308.
[8] 岳秀梅.淡竹叶中总黄酮的提取与含量测定[J].世界中西医结合杂志.2009,4(3):177-178.
[9] 卫静莉,高松平,董梅,等.柿叶提取黄酮类化合物方法及鉴定[J].2007,21(3):47-49.
[10] 赵翾,李红良,张惠妹.响应面法优化火麻仁黄酮提取工艺[J].食品科学,2011, 32(2): 127-132.
[11] 张国文,胡明明,何力,等.响应面分析法优化微波提取夏枯草中黄酮化合物的工艺研究[J]. 南昌大学学报(工科版), 2010,32(4):385-389.
[12] 于浩,阮美娟,赵龙,等.响应面法优化荷叶黄酮超声提取条件的研究[ J].食品研究与开发,2010,31(1):55-60.
[13] 吴华勇,黄赣辉,顾振宇,等.响应曲面法优化竹叶总黄酮的提取工艺研究[J].食品科学, 2008,29(11):196-200.
[14] Ji Young Lim, Hyang-Sik Yoon, Kwang-Yup Kim.Optimum conditions for the enzymatic hydrolysis of citron waste juice using response surface methodology (RSM)[J]. Food Science and Biotechnology,2010,19(5):1135-1142.
[15] 周丽娜,翁德宝.菊花脑多糖及黄酮类化合物分离提取的研究[J].江苏教育学院学报(自然科学版),2005,22(3):12-15.