Optimizing Pectinase Hydrolysis Conditions of Strawberry Pulp by Response Surface Methodology

Abstract

Abstract: The aim of this study was to develop the best process for hydrolyzing strawberry pulp with pectinase. Based on one-factor-at-a-time experiments, a three-factor and three-level Box-Behnken central composite design coupled with response surface analysis was employed to explore the effects of enzyme concentration, hydrolysis temperature and hydrolysis time on the yield of strawberry syrup. The results indicated that the optimal extraction conditions were achieved hydrolysis at 45 ℃for 5 h with 13.38 mg/L of enzyme. Under these optimal conditions, the predicted value of juice yield was 84.69%, while the actual value was 84.75%, suggesting a good fitting between the developed regression equation and actual values.

Key words: strawberry pulp, response surface methodology, pectinase, juice yield

CLC Number:

TS255.46

References

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