Optimization of Enrichment Medium for Bifidobacterium longum by Response Surface Methodology

doi:10.7506/spkx1002-6630-201305043

Abstract

Abstract: In order to improve viable Bifidobacterium longum, the optimization of enrichment medium was carried out by response surface methodology. The optimal carbon source for Bifidobacterium longum was lactose. Xylooligosaccharide, inulin, isomaltooligosaccharide, fructooligosaccharide, phenylalanine, methionine, proline, glutamic acid and lysine were found to significantly promote the growth of Bifidobacterium longum. Three most important nutrients for Bifidobacterium longum were screened via Plackett-Burman using software of Design expert 8.06. Optimal concentrations of three substrates were confirmed by Box-Behnken and response surface analysis to be 1.7 g/L of xylooligosaccharide, 3.6 g/L of inulin and 0.4 g/L proline. Cultivated in optimal enrichment medium, the viable counts of Bifidobacterium longum after 18 h reached up to (1.75 ± 0.02)×109 CFU/mL, which revealed an increase by 95.64% when compared with the original medium.

Key words: Bifidobacterium longum, enrichment medium, response surface methodology, prebiotics, amino acid

CLC Number:

TS201.3

SUN Peng 1，ZHAO Xu-bo2，SUN Xian-feng1，MA Yong-zheng3. Optimization of Enrichment Medium for Bifidobacterium longum by Response Surface Methodology[J]. FOOD SCIENCE, 2013, 34(5): 207-212.

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