Characterization of Enzymatic Properties of High Homoserine Dehydrogenase-Producing Mutant D206G from
Corynebacterium pekinense AS1.299

doi:10.7506/spkx1002-6630-201307050

Abstract

Abstract: Homoserine dehydrogenase (HSD), a key enzyme involved in the biosynthesis of the aspartic acid family of amino acids, plays an important role in the synthesis of threonine and methionine. High HSD-producing mutant D206G from Corynebacterium pekinense AS1.299 was successfully constructed and heterologously expressed in E.coli BL21(DE3). Molecular docking and homologous alignment demonstrated that site 206 was a highly conservative sequence bound with substrate. The optimal pH, temperature and half-life of HSD from the mutant were 7.5, 37 ℃ and 4 h, respectively. In addition, it showed high resistance to metal ions and organic solvents. Compared with its wild-type counterpart, the Km of mutant D206G decreased, possibly due to a reduction in side-chain functional groups and consequent decrease in steric hindrance, thus promoting the enzyme-substrate binding.

Key words: Corynebacterium pekinense, homoserine dehydrogenase, site-directed mutagenesis, enzymatic

CLC Number:

Q814.9

XU Jin-kun1,2，MIN Wei-hong1,2,*，ZHAN Dong-ling1,2，FANG Li1,2，LIU Jia1,2，SHEN Shu-xia1,2，GUO Yong-ling1,2. Characterization of Enzymatic Properties of High Homoserine Dehydrogenase-Producing Mutant D206G from Corynebacterium pekinense AS1.299[J]. FOOD SCIENCE, 2013, 34(7): 240-244.

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Characterization of Enzymatic Properties of High Homoserine Dehydrogenase-Producing Mutant D206G from Corynebacterium pekinense AS1.299

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