Development of Colloidal-Gold Immunochromatographic Strip for Rapid and Quantitative Detection of Clenbuterol in Swine Urine

doi:10.7506/spkx1002-6630-201312024

Abstract

Abstract:

We report on the development of a rapid and quantitative colloidal gold immunochromatographic strip for
clenbuterol (CLE) detection in swine urine. The parameters that influenced the assay performance of immunochromatographic
strip were optimized by an orthogonal array design L9 (3)3 and a quantitative method based on T/C ratio was developed to
detect CLE residue in swine urine. The limit of detection (LOD) of immunochromatographic strip for CLE in swine urine was
0.22 ng/mL and a linear calibration curve was obtained over the concentration range of 0.1 to 2.0 ng/mL with a half maximal
inhibitory concentration (IC50) of 0.36 ng/mL. For spiked blank urines, mean recoveries were (102.4 ± 7.8)% at 0.5 ng/mL,
(96.5 ± 6.8)% at 1.0 ng/mL and (103.3 ± 7.2)% at 1.5 ng/mL, respectively. In conclusion, we have successfully developed a
rapid and quantitative method based on colloidal gold immunochromatographic strip for CLE residue in swine urine.

Key words: immunochromatographic strip, clenbuterol, quantitative detection

LI Chao-hui，LUO Wei，XU Bo，LIU Wen-juan，XU Heng-yi，XIONG Yong-hua. Development of Colloidal-Gold Immunochromatographic Strip for Rapid and Quantitative Detection of Clenbuterol in Swine Urine[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201312024.

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Development of Colloidal-Gold Immunochromatographic Strip for Rapid and Quantitative Detection of Clenbuterol in Swine Urine

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