Determination of Four Arsenic Species in Bee Pollen by High Performance Liquid Chromatography-Hydride Generation-Atomic Fluorescence Spectrometry

Abstract

Abstract:

This paper describes a high performance liquid chromatography- hydride generation-atomic fluorescence
spectrometry (HPLC-HG-AFS) method for the determination of arsenite (AsⅢ), monomethylarsonic acid (MMA),
dimethylarsinic acid (DMA) and arsenate (AsⅤ) in bee pollen. Sample preparation was achieved by ultrasonic extraction
with 0.20 mol/L sulfuric acid. The detection limits for AsⅢ, DMA, MMA and AsⅤ were 1.0, 2.1, 1.2 and 3.1 μg/kg,
respectively, and recoveries at two spiked levels were in the range of 79%–100% with relative standard deviations (RSDs)
of 4.5%, 2.3%, 11.0% and 7.7%, respectively. In conclusion, we have established a good, fast qualitative and quantitative
method for analysis of arsenic compounds in bee pollen with high sensitivity and good accuracy.

Key words: bee pollen, arsenic species, high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS)

WANG Su-fen，CHEN Fang，WANG Peng，WU Li-ming. Determination of Four Arsenic Species in Bee Pollen by High Performance Liquid Chromatography-Hydride Generation-Atomic Fluorescence Spectrometry[J]. FOOD SCIENCE.

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Determination of Four Arsenic Species in Bee Pollen by High Performance Liquid Chromatography-Hydride Generation-Atomic Fluorescence Spectrometry

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