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Lotus Bee Pollen Polysaccharides Significantly Relieve Intestinal Mucosal Barrier Damage in Mice Caused by Fluorouracil

FANG Xiaoming1,2, TIAN Wenli2, ZHANG Xiaolin1, PENG Wenjun2, XIAO Hongwei1, GAO Lingyu2, WANG Canhong3, GAO Zhenjiang1,*   

  1. 1. College of Engineering, China Agricultural University, Beijing 100083, China; 2. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
  • Online:2016-08-15 Published:2016-08-30


Objective: To investigate the protective effects of polysaccharides (LP) extracted from lotus bee pollen dried
by different drying methods on intestinal mucosal inflammation and intestinal barrier dysfunctions in mice induced by
5-fluorouracil (5-Fu). Methods: Mouse models of intestinal mucosal inflammation were established by induction with 5-Fu
and the model mice were grouped randomly and subjected to drug administration. The carbon-propelling rate was tested,
and colon length and Peyer’s patches (PPs) area were measured. The colon tissue was stained with hematoxylin-eosin (HE)
for histological section observation. Reactive oxygen species (ROS) and glutathione (GSH) contents, and catalase (CAT) and
GSH-Px activities in the supernatant of PPs homogenate were evaluated by biochemical assays. Tumor necrosis factor-α (TNF-α)
in the supernatant of colon tissue homogenate was determined through ELISA. Results: Compared with the normal group,
intraperitoneal injection of 5-Fu to mice significantly inhibited small intestinal transit (P < 0.05). LP extracted from lotus bee pollen
dried by hot air or vacuum pulsed drying improved colon shortening caused by 5-Fu (P < 0.05) and resulted in a significant or highly
significant increase of PPs area (P < 0.05 or P < 0.01). Moreover, compared with the 5-Fu-induced model group, LP combined with
5-Fu could significantly promote intestinal transit (P < 0.05), increase GSH content, and CAT and GSH-Px activities (P < 0.01 or
P < 0.05), and decrease obviously TNF-α contents (P < 0.05 or P < 0.01). HE staining results showed that LP could significantly
reduce the loss of colonic mucosa epithelium goblet cells, inflammatory cell infiltration and more shallow crypt depth induced
by 5-Fu. Conclusion: LP could relieve intestinal mucosal damage, improve intestinal transit dysfunction and protect against
intestinal oxidative damage in mice induced by 5-Fu. The polysaccharides extracted from lotus bee pollen subjected to vacuum
pulsed drying exhibited better activity than the polysaccharides extracted from hot air dried lotus bee pollen.

Key words: lotus bee pollen polysaccharide, 5-fluorouracil, intestinal mucosal barrier, oxidative damage, drying method

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