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Optimization of Solid-state Fermentation of Rice by Cordyceps militaris by Response Surface Methodology

WANG Yong-xian1,LIU Yan1,JIANG Peng1,JIANG Jian-jian1,ZHU Yi-ming1,MA Li-kun2   

  1. 1. Qingdao Edible Fungus Workstation, Qingdao 266071, China;2. Qingdao Seed Service Center, Qingdao 266071, China
  • Online:2013-10-15 Published:2013-09-27
  • Contact: WANG Yong-xian

Abstract:

Plackett-Burman design was applied to screen 3 significant ones (material/water ratio, light intensity and
illumination time) from 5 potential key factors influencing the solid-state fermentation of rice by Cordyceps militaris. After
the maximum response region was approached by steepest ascent path design, the significant factors were optimized by
central composite design combined with response surface methodology. A material/water ratio of 5.19:1 (m/V), temperature
of 25 ℃, inoculum size of 12 mL/100 g, light intensity of 980.13 lx and an illumination time of 9.19 d were found to the
optimum conditions to obtain a cordycepin yield of 64.1 mg/100 g.

Key words: response surface methodology, Cordyceps militaris fermented rice, cordycepin

CLC Number: