A Novel Method of Nano-Immunomagnetic Separation-Real Time-Polymerase Chain Reaction for Detecting Vibrio parahaemolyticus in Seafoods

doi:10.7506/spkx1002-6630-201404022

Abstract

Abstract:

A novel method using monoclonal antibody of Vibrio parahemolyticus, nano-immunomagnetic separation (Nano-
IMS) plus real-time polymerase chain reaction (RT-PCR) was established for detecting Vibrio parahemolyticus in seafoods.
The nano-immunomagnetic beads (Nano-IMB) created showed a capture rate for Vibrio parahemolyticus of 74% at a
bacterial concentration of 103 CFU/mL. The sensitivity of Nano-IMS-PCR was 140 CFU/mL under pure culture conditions
without enrichment. The RT-PCR method was highly specific for 134 Vibrio parahemolyticu and 74 non-target bacteria. The
detection limit of the method reached 2 CFU/25 g in artificial food samples. The enrichment time was shortened to 8 h.

Key words: Vibrio parahaemolyticus, nano-immunomagnetic separation, real time-polymerase chain reaction

CLC Number:

TS207.4

ZHANG Lei1,2, ZENG Jing1,*, WEI Hai-yan1, MA Dan1, CHENG Jin-xia2, ZHANG Xi-meng1, ZHANG Hai-yu2. A Novel Method of Nano-Immunomagnetic Separation-Real Time-Polymerase Chain Reaction for Detecting Vibrio parahaemolyticus in Seafoods[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201404022.

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A Novel Method of Nano-Immunomagnetic Separation-Real Time-Polymerase Chain Reaction for Detecting Vibrio parahaemolyticus in Seafoods

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