Abstract:

Propolis is generally made up of 50% plant resins, and its variety depends on plant origins in the location where
honeybees collecte propolis. In this study, it was observed that honeybee workers collected resin from the bud of Populus
canadensis Moench, carried the red resin with their hind legs and then removed the pieces of red resin inside the hive.
Furthermore, the bud resin of Populus canadensis Moench, resinous substance collected from honeybees’ hind legs and
propolis in bee houses were extracted separately with aqueous ethanol solvent for the analysis of polyphenolic components
via high performance liquid chromatography (HPLC). The results suggested that their similarity displayed not only in
physical properties but also chemical components as well as relative content of each component. Thus, the plant origin of
Chinese propolis can be Populus canadensis Moencha, a hybrid variety of Populus nigra L.

Key words: high performance liquid chromatography, propolis, plant origin, bud resin of Populus canadensis Moench