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Construction of Fruit Specific Expression Vector pBI121-2A11-HB-1 and Its Transformation to Agrobacterium

YE Hong-hong, YIN Ming-an*, ZHU Min, REN Xiao-lin   

  1. College of Horticulture, Northwest A&F University, Yangling 712100, China
  • Online:2014-10-15 Published:2014-10-17

Abstract:

ACO is a key enzyme in the biosynthesis of ethylene. LeHB-1 is the transcription factor of tomato gene ACO1. By
studying the activity of transcription factor LeHB-1, one can understand the regulation mechanism of ethylene synthesis. In
this study, the sequences of fruit-specific promoter 2A11 and LeHB-1 gene were cloned from tomato by PCR. Then the sense
and antisense fruit-specific expression vectors of LeHB-1 were constructed and transformed into Agrobacterium GV3101, and
then the plant with the transformation of targeted bacteria was obtained. This research lays a foundation for further study of the
biological functions of the transcription factor LeHB-1 during the development of fruits via the transformation of tomato.

Key words: tomato, specific expression vector pBI121-2A11-HB-1, vector construction, Agrobacterium-mediated genetic transformation system

CLC Number: