Purification and Separation of Pu-Erh Tea Polysaccharide by Resin

doi:10.7506/spkx1002-6630-201502004

FOOD SCIENCE

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Purification and Separation of Pu-Erh Tea Polysaccharide by Resin

YANG Xinhe1,2, HUANG Jian’an2, LIU Zhonghua1,2,*, MAO Qingli1,2

1. School of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China;
2. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China

Online:2015-01-25 Published:2015-01-16
Contact: LIU Zhonghua

Abstract

Abstract:

Among ten types of macroporous resins, D101 resin was selected as the best for the decolorization and
deproteinization of Pu-Erh tea polysaccharides. When 50 mL of the sample at a concentration of 3.8 mg/mL, pH 4 was
adsorbed by 11 mL of D101 resin 50 ℃, the decolorization rate was 82.33% and the removal rate of protein was 70.89%.
Six polysaccharide fractions were obtained after subsequent DEAE-52 cellulose column chromatography.

Key words: resin, Pu-Erh tea, polysaccharide, purification, separation

CLC Number:

S571.1

YANG Xinhe1,2, HUANG Jian’an2, LIU Zhonghua1,2,*, MAO Qingli1,2. Purification and Separation of Pu-Erh Tea Polysaccharide by Resin[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201502004.

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Purification and Separation of Pu-Erh Tea Polysaccharide by Resin

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