Screening and Identification of Se-Enriching Lactic Acid Bacteria

doi:10.7506/spkx1002-6630-201503034

Abstract

Abstract:

A total of 15 lactic acid bacteria derived from 5 species were studied by comparing their growth curves and
abilities to tolerate and enrich Se. We also identified and characterized the selected strain by biochemical and molecular
biological methods. The results showed that the optimum time to add Se was at the end of 6-hour culture period after which
the culture was carried out for another 18 hours. The optimum sodium selenite concentration for Se enrichment was found to
be 6 μg/mL by comparing 9 selected strains. The strain BC-25 was eventually selected as the best Se-enriching strain, which
could enrich 425.79 μg/g of Se with a transformation rate of 27.00%. The 16S rDNA sequence results indicated that BC-25
is Lactobacillus plantarum.

Key words: lactic acid bacteria, Se enrichment, screening, identification

CLC Number:

TS254

ZENG Yiting, GUO Xilang, ZHOU Kang*, LIU Shuliang, HAN Xinfeng. Screening and Identification of Se-Enriching Lactic Acid Bacteria[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201503034.

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Screening and Identification of Se-Enriching Lactic Acid Bacteria

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