Abstract:

A gas chromatography-mass spectrometry (GC-MS) combined with NAGINATA™ software method was
developed for screening multi-pesticide residues in edible fungi. The pesticide residues in samples were extracted with
acetonitrile, cleaned up with Carbon-NH2 solid-phase extractor, detected by GC-MS, and finally analyzed by NAGINATA™
software. Based on the quality precision and retention time of each compound, the possible hazardous chemical compounds
were screened out by matching mass spectra with mass spectral library. Under the optimal conditions, the limits of detection
(LOD) of the established method were in the range of 0.001–0.050 mg/kg, and the average recovery rates for 18 pesticide
residues spiked in a blank sample were 77%–110% with relative standard deviations (RSDs) of 1.1%–6.9%. The method was
applied on 35 edible fungi purchased from local markets, and two pesticides were detected. which were below the maximum
residue limit (MRL) stipulated by the Chinese national standard GB 2763—2014. This method proved to be rapid, simple,
efficient and suitable for the fast screening of pesticide residues in edible fungi.

Key words: edible fungi, NAGINATA&trade, software, solid-phase extraction, gas chromatography-mass spectrometry (GC-MS), pesticide residues