Screening of Lactic Acid Bacteria with High Protease and Lipase Activities from Xinjiang Traditional Yogurt

doi:10.7506/spkx1002-6630-201517025

Abstract

Abstract:

This study aimed to screen lactic acid bacteria with high protease and lipase activities from the traditional
yogurt in Xinjiang Province, China. Lactic acid bacterial strain RA3 with protease activity of 38.87 U/mL was screened
by transparent circle method, and a lipase producing-lactic acid bacterial strain RC4 with lipase activity of 8.54 U/mL was
screened by copper soap method. The strains RA3 and RC4 were identified as Lactobacillus fermentum and Lactobacillus
plantarum, respectively, using API 50 CHL physiological and biochemical kit and 16S rRNA sequence alignment. Both
strains can provide new starter cultures for fermented foods.

Key words: lactic acid bacteria, screening, protease activity, lipase activity

CLC Number:

TS252.42

HE Jie, ZENG Xiaoqun, Lü Mingchun, MAO Zhongxuan, WANG Xianglin, PAN Daodong. Screening of Lactic Acid Bacteria with High Protease and Lipase Activities from Xinjiang Traditional Yogurt[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201517025.

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Screening of Lactic Acid Bacteria with High Protease and Lipase Activities from Xinjiang Traditional Yogurt

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