Identifying Fish Products in Shenzhen through DNA Barcoding

doi:10.7506/spkx1002-6630-201520048

FOOD SCIENCE

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Identifying Fish Products in Shenzhen through DNA Barcoding

WANG Min1, LIU Hong2, HUANG Hai3, ZHAO Xiaomeng1, SHI Qiong1, HE Shunping1,4, SUN Ying1,*

1. BGI-Shenzhen, Shenzhen 518083, China; 2. Animal & Plant Inspection and Quarantine Technology Center,
Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China;
3. Sanya Science and Technology Academy for Crop Winter Multiplication, Sanya 572000, China;
4. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Online:2015-10-25 Published:2015-10-20
Contact: SUN Ying

Abstract

Abstract:

In this study, DNA barcoding was applied to identify the exact species of fish products from Shenzhen wholesale
and retail markets and to check if these products are correctly labeled. Using mitochondrial cytochrome C oxidase subunit
Ⅰ (COⅠ ) gene as the target, the sequences of COⅠ from different samples were analyzed. Our results showed that the
COⅠ gene sequences of all 77 samples examined were successfully amplified by PCR. However, up to 36.36%（28/77）
of them were inconsistent with their label descriptions, in which all the products labeled with “tongue fish” were actually the
low-cost Pangasianodon hypophthalmus. As a simple, rapid and efficient technology, DNA barcoding can be widely used for
species identification of fish products.

Key words: DNA barcoding, mitochondrial cytochrome C oxidase subunit Ⅰ (COⅠ) gene, species identif??ication, fish products

CLC Number:

WANG Min1, LIU Hong2, HUANG Hai3, ZHAO Xiaomeng1, SHI Qiong1, HE Shunping1,4, SUN Ying1,*. Identifying Fish Products in Shenzhen through DNA Barcoding[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201520048.

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Identifying Fish Products in Shenzhen through DNA Barcoding

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