DNA Metabarcoding in Food Species Identification: Current Applications and Future Prospects

doi:10.7506/spkx1002-6630-201813042

Abstract

Abstract: Food species identification is one of the most important aspects in food authenticity assessment. Polymerase chain reaction and gene chip technology have been applied for food species identification. High-throughput sequencingbased metabarcoding technology has developed rapidly in recent years. Compared with the standard Sanger sequencingbased DNA barcoding approach, metabarcoding has shown great advantages in food species identification due to its low cost, high throughput and rapidity for the simultaneous detection of multiple species in complex samples. This review presents the definition of metabarcoding and the methods used in this research field. Then, we summarize the applications and challenges of metabarcoding in food species identification. Finally, we discuss the future direction for the development of metabarcoding.

Key words: DNA barcoding, high-throughput sequencing, metabarcoding, food species identification

CLC Number:

TS207.3

XING Ranran, WU Yajun, CHEN Ying. DNA Metabarcoding in Food Species Identification: Current Applications and Future Prospects[J]. FOOD SCIENCE, 2018, 39(13): 280-288.

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DNA Metabarcoding in Food Species Identification: Current Applications and Future Prospects

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