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Comparative Analysis of Quantitative Assay Methods for β-D-Glucan from Yeast Cell Walls

TIAN Xiaoli1,2, YANG Ping1,*, JIANG Wenxia1,*   

  1. 1. Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Key Laboratory of Systems Microbial
    Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;
    2. College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
  • Online:2016-01-25 Published:2016-01-15
  • Contact: TIAN Xiaoli, YANG Ping

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Abstract:

This study was conducted to compare and analyze several quantitative assay methods for insoluble β-D-glucan
from yeast cell walls. It was indicated that the sample pretreatment by acid hydrolysis provided higher results for the determination
of β-D-glucan, while the results obtained by acid-enzymatic hydrolysis were more precise. Among different test methods for
glucose obtained after hydrolysis, the biosensor method was found to be faster, more stable and suitable for quality control in the
production process. Besides, α-glucan in the samples had a significant interference effect on the results of determination. Thus β-Dglucan
should be calculated by subtraction from total glucose of the glucose obtained from α-glucan.

Key words: β-D-glucan, yeast cell walls, hydrolysis method, quantitative determination, biosensor method

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