Abstract:

The supercritical-CO2 fluid extraction conditions of volatile oil from dried raw ginseng were optimized by
response surface methodology. Chemical constituents of the essential oil were analyzed by gas chromatography-mass
spectrometry (GC-MS) combined with automated mass spectral deconvolution and identification system (AMDIS) based
on retention index. The optimal extraction conditions were determined as follows: extraction pressure, 38 MPa; extraction
temperature, 55 ℃; static extraction time, 2 h and dynamic extraction, 1 h, under which, the maximum oil yield of 1.12%
was obtained. A total of 35 chromatographic peaks were separated from the essential oil of ginseng roots, 29 of which were
identified, accounting for 96.69% of the total peak area.

Key words: ginseng essential oil, supercritical fluid extraction, response surface methodology, automated mass spectral deconvolution and identification system (AMDIS), retention index