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Detection of Genetically Modified Soybean by Helicase-Dependent Isothermal DNA Amplification Assay

ZHANG Jingjing1, WANG Zan1, ZHANG Cuixia1, MA Chaofeng1, ZHOU Wei1,2,*, ZHANG Yan1   

  1. 1. Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China;
    2. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China
  • Online:2016-02-25 Published:2016-02-23
  • Contact: ZHOU Wei

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Abstract:

Objective: To establish a new rapid method to detect genetically modified soybean based on helicase-dependent
isothermal DNA amplification (HDA). Methods: With four highly specific sets of primers synthesized to target genes
including Lectin in soybean and CaMV35S, NOS and CP4-EPSPS foreign genes, PCR reaction system was established. The
specificity and sensitivity of the PCR method were tested based on HDA and homology analysis was performed. Results: The
helicase-dependent isothermal DNA amplification method for the rapid detection of genetically modified soybean has been
established. The detection limits of CaMV35S, NOS and CP4-EPSPS genes in genetically modified soybeans by HDA were
all 0.2%. Conclusion: HDA is a rapid, user-friendly, specific and sensitive method for the detection of genetically modified
soybean, and is very suitable for use in grassroots laboratories with a broad prospect.

Key words: helicase-dependent isothermal DNA amplification (HDA), genetically modified soybean, detection

CLC Number: