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Development of A Multi-Residue Electrochemical Immunoassay Based on Broad-Spectrum Specific Antibody for Determination of β2-Agonists

REN Pengfei1, SHAO Kefeng1, ZHANG Jie1, ZHANG Honglin2, ZHAO Bo1,*   

  1. 1. Jiangsu Collaborative Innovation Center of Biomedical Functional Material, Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China;
    2. College of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China
  • Online:2016-04-25 Published:2016-04-13

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Abstract:

Salbutamol-ractopamine-bovine serum albumin conjugate (SAL-RAC-BSA) was prepared and served as
immunogen to obtain a broad-spectrum specific antibody (anti-SAL-RAC-BSA Abs) through immunization of rabbits.
Electrochemical immunosensor modified with reduced graphene oxide-chitosan composite and SAL was constructed and
its electrochemical behavior was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Indirect
competitive electrochemical immunoassay was carried out for the simultaneous determination of three β2-agonis, including
clenbuterol (CL), salbutamol (SAL) and terbutaline (TB). The proposed method indicated a linearity in the range from 50 to
7 000 ng/mL, 1 to 1 500 ng/mL and 100 to 6 000 ng/mL for CL, SAL and TB, respectively, and the limits of detection were
0.2, 0.3 and 0.3 ng/mL, respectively. The recovery rates of clenbuterol in spiked pork, fat and liver samples ranged from
84.7% to 106%. In summary, a sensitive, rapid and simple electrochemical immunoassay was successfully developed for the
determination of three β2-agonists.

Key words: broad-spectrum specific antibodies, β2-agonists, multi-residue, electrochemical immunosensor

CLC Number: