Progress in Hybrid-Hybridoma Technology to Produce Bispecific Monoclonal Antibody and Its Application in Food Safety Detection

doi:10.7506/spkx1002-6630-201613045

Abstract

Abstract:

Bispecific monoclonal antibodies (BsMAb) are monoclonal antibody molecules with two different specific
antigen-binding sites. Hybrid-hybridoma technology has a great advantage in the preparation of BsMAb because of its easy
operation and less time consumption. BsMAbs with 2 distinct antigen-binding sites produced by the hybrid-hybridoma
technology could specifically recognize antigens of 2 drugs or 2 classes of drugs, and are applicable in the multi-residue
determination of foods. In this paper, we elaborate recent progress in the development of hybrid-hybridoma technology and
explore the mechanism of BsMAb generation by the hybrid-hybridoma method. Several important factors influencing the
hybrid-hybridoma technology are discussed, and the application of BsMAb in food safety detection is also reviewed. This
paper will provide some references for the development and application of BsMAb in multi-residue immunoassay of foods.

Key words: hybrid-hybridoma technology, bispecific monoclonal antibody (BsMAb), multi-residue, immunoassay

CLC Number:

TS207.3

WANG Feng, WANG Yu, WANG Hong, XIAO Zhili. Progress in Hybrid-Hybridoma Technology to Produce Bispecific Monoclonal Antibody and Its Application in Food Safety Detection[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613045.

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