Abstract:

4,4-Bis (4-hydroxyphenyl) valeric acid (BVA), a derivative of bisphenol A (BPA), was chosen as the hapten to
prepare the artificial antigen. An active ester method was used for coupling BPA hapten with keyhole limpet hemocyanin
(KLH) and ovalbumin (OVA), respectively. BALB/c mice were immunized with the immune antigen to produce antibody.
After cell fusion with PE G method, the hybridoma cell lines generating anti-BPA monoclonal antibodies were obtained. The
monoclonal antibody was identified as IgG1 subtype with κ light chain. An indirect competitive enzyme-linked immunoassay
(ELISA) was set up using the monoclonal antibody. The linear range of the inhibition curve was between 1 and 50 ng/mL.
The limit of detection was 0.43 ng/mL, and the IC50 value was 6.56 ng/mL. The recovery rates of BPA in water ranged from
82.83% to 101.94% with variation coefficients of 2.94%–12.95%.The proposed immunoassay offers a great potential for
sensitive and specific detection of BPA in food safety monitoring.

Key words: bisphenol A, monoclonal antibody, enzyme-linked immunoassay