Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G

doi:10.7506/spkx1002-6630-201614013

Abstract

Abstract:

The hybridoma cells secreting monoclonal antibody (McAb) against bovine colostrum immunoglobulin G (IgG)
were developed by cell fusion technology after immunization of BALB/c mice with bovine IgG. McAbs were obtained by
purification of mouse ascites. A double antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique was
established for the detection of bovine colostrum IgG. The linear range was 7.8–1 000 ng/mL and the limit of detection (LOD)
was 7.06 ng/mL. The intra-assay and inter-assay coefficient of variations were 4.52% and 4.94%, respectively. The recovery
rate was 91.85%–102.45%. The ELISA method can provide a simple, accurate and stabile approach for the rapid detection
of bovine colostrum IgG.

Key words: bovine colostrum, immunoglobulin G (IgG), monoclonal antibody, sandwich ELISA

CLC Number:

TS252.7

LIU Jin, WANG Liwei, YUE Xiqing. Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614013.

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