Abstract:

The acute and genetic toxicity of whole powder of Viscum liquidambaricolum Hayata were evaluated using acute
toxicity, bone marrow polychromatic erythrocyte micronucleus, mouse spermatogonium chromosome aberration and Ames
tests as described in the Chinese national standard Toxicological Evaluation Procedure for Food Safety (GB 15193.1?2014).
The oral acute toxicity test revealed that the medium lethal dose (LD50) of the powder was 10.25 g/kg for both male and
female rats, which suggested that it was actually non-toxic. Both bone marrow polychromatic erythrocyte micronucleus
and Ames tests gave negative results. Spermatogonium chromosome aberration tests at 10.25 and 5.12 g/kg doses revealed
temporarily high aberration rate, which was not observed in the 2.57 g/kg group. Therefore, whole powder of Viscum
liquidambaricolum Hayata showed neither acute nor genetic toxicity.

Key words: Viscum liquidambaricolum Hayata, acute toxicity, bone marrow polychromatic erythrocyte micronucleus test, spermatogonium chromosome aberration test, Ames test, whole powder