Construction of Genetically Engineered E. coli and Optimization of Its Fermentation Conditions for High-Level Production of Indigo

doi:10.7506/spkx1002-6630-201623031

Abstract

Abstract: A genetically engineered strain of Escherichia coli for the expression of the styrene monooxygenase gene from
Pseudomonas putida was constructed by recombinant DNA technique, and the fermentation conditions of the recombinant
strain were optimized for enhanced indigo production. Firstly, the effects of four factors including substrate concentration,
fermentation temperature, initial pH and inoculum amount on indigo production were investigated. Then, the response surface
methodology was applied to optimize the variables showing significant influence on indigo production, substrate concentration,
fermentation temperature and initial pH. Results revealed that the optimized fermentation conditions were determined as substrate
concentration of 0.95 mg/mL, fermentation temperature of 29 ℃ and initial pH 6.9. The predicted indigo production was
67.65 mg/mL, which was in close agreement with the predicted value, indicating that the model was feasible.

Key words: indigo, styrene monooxygenase, genetically engineered strain, fermentation conditions, response surface methodology

CLC Number:

TS201.3

CHENG Lei, YIN Sheng, SUN Baoguo, CHEN Min, WANG Chengtao, LONG Xinran, WANG Zihao. Construction of Genetically Engineered E. coli and Optimization of Its Fermentation Conditions for High-Level Production of Indigo[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201623031.

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Construction of Genetically Engineered E. coli and Optimization of Its Fermentation Conditions for High-Level Production of Indigo

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