Abstract: The aim of this research was to determine the synergistic effect of ultra-high pressure and enzymatic treatments on allergen elimination from shelled shrimp (Litopenaeus vannamei). Fresh shrimp were treated by ultra-high pressure alone and in combination with enzymatic digestion after removing head, tail, shell, and digestive tract. The allergenicity of the treated samples was examined by indirect enzyme-linked immunosorbent assay (ELISA). Results showed that the highest percentage of allergen elimination of 45.66% was obtained by single ultra-high pressure treatment under 600 MPa at 40 ℃ for 30 min, and it was increased to 95.27% by combining ultra-high pressure treatment under 200 MPa for 30 min at 40 ℃, enzyme dosage 3 000 U/mg with trypsin hydrolysis. Therefore, the allergenicity of shelled L. vannamei could be eliminated by ultra-high pressure treatment, and better effect could be achieved by combination with enzymatic hydrolysis.

Key words: Litopenaeus vannamei, combined ultra-high pressure and enzymatic treatments, trypsin, allergen elimination