Optimization of Extraction Process for Crude Polysaccharides from Stropharia rugoso-annulata by Response Surface Methodology

doi:10.7506/spkx1002-6630-201202030

Abstract

Abstract: The optimal extraction process for crude polysaccharides from stropharia rugoso-annulata was investigated by response surface methodology to explore the effects of extraction temperature, extraction time, extraction repeat number and solid-liquid ratio on the yield of crude polysaccharides. Based on the single-factor tests, Box-Behnken design with three key factors was conducted and a regression model was established. The results showed that optimal extraction process parameters were extraction temperature of 75 ℃, extraction time of 20 min and solid-liquid ratio of 1:25 (g/mL). Under the optimal extraction conditions, the yield of crude polysaccharides from Stropharia rugoso-annulata was up to 7.614%.

Key words: Stropharia rugoso-annulata, crude polysaccharides, response surface methodology (RSM)

CLC Number:

TS205.9

CHEN Jun-chen, LAI Pu-fu, ZHOU Xue-hua, WENG Min-jie, LI Yi-bin. Optimization of Extraction Process for Crude Polysaccharides from Stropharia rugoso-annulata by Response Surface Methodology[J]. FOOD SCIENCE, 2012, 33(2): 139-142.

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Optimization of Extraction Process for Crude Polysaccharides from Stropharia rugoso-annulata by Response Surface Methodology

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