Identification of Triacylglycerols in the Lipids of Pseudosciaena crocea by UPLC and Q-Exactive High-Resolution
Mass Spectrometer

doi:10.7506/spkx1002-6630-201710030

Abstract

Abstract: The triglyceride composition of Pseudosciaena crocea lipids was analyzed by ultra performance liquid chromatography-electrospray ionization/quadrupole/electrostatic field orbit trap high resolution mass spectrometry (UPLCESI/ Orbitrap-MS). The chromatographic separation was performed on a Hypersil Gold C18 column (150 mm × 4.6 mm, 2.1 μm) using a mobile phase consisting of methanol (including 0.1% formic acid)-isopropyl alcohol (82:18, V/V) and 0.05% ammonium acetate. The analytes were detected by ESI/Orbitrap-MS in the positive ion mode to obtain mass and tandem mass spectra together and then the results obtained were compared with those from the Lipidblast database. A total of 28 triacylglycerols were identified by UPLC-ESI/Orbitrap-MS). Among these, PoOL was the most abundant (35.261%), and PoOL, PoSO, PPO, PPoO, PSO, MPO, POL and PoSA together accounted for 87.87% of the total triacylglycerols.

Key words: Pseudosciaena crocea, lipid, fatty acid, triacylglycerols, electrospray ionization

CLC Number:

S912

WENG Liping , ZHAO Yun , ZHANG Le , WANG Honghai. Identification of Triacylglycerols in the Lipids of Pseudosciaena crocea by UPLC and Q-Exactive High-Resolution Mass Spectrometer[J]. FOOD SCIENCE, 2017, 38(10): 178-182.

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Identification of Triacylglycerols in the Lipids of Pseudosciaena crocea by UPLC and Q-Exactive High-Resolution Mass Spectrometer

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