Abstract: In this paper, a novel disposable centrifugal microfludic chip with pre-storage biochemical reagents was developed for the rapid detection of pesticide residues in agricultural products, which was based on the principle of enzyme inhibition and photometric analysis. The microfludic chip was integrated with sampling, enzyme inhibition, color development and detection chamber. By coupling with a portable detection device developed in our laboratory, the microfludic chip could provide a simple, easy-to-use, low-cost and sensitive approach for rapid detection of organophosphate and carbamate pesticides on site. Results indicated that the microfluidic chip could achieve an automatic detection process with the advantages of less consumption of reagents (reduction by one-twentieth) and satisfied accuracy compared with the traditional pesticide residue detector. Furthermore, the microfluidic chip was proved to be a powerful tool for screening pesticide residues in the case of high-throughput requirement. The microfluidic chip was superior to the traditional pesticide residue detector in term of detection sensitivity, reproducibility and measurement accuracy. The microfluidic chip coupled with self-made detection device is particularly suitable for on-site, rapid and high-throughput screening of pesticide residues by nonprofessionals.

Key words: microfluidic chip, pesticide residues, rapid detection, acetylcholin esterase (Ache) inhibition method