FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (21): 15-20.doi: 10.7506/spkx1002-6630-201721003

• Basic Research • Previous Articles     Next Articles

Inhibitory Effect of Isothiocyanate Flavors on Two Gram-Positive Pathogenic Bacteria

WANG Yan, ZHOU Qianqian, WAN Xiulin, WANG Jiaying, HOU Hongman, ZHANG Gongliang   

  1. (School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)
  • Online:2017-11-15 Published:2017-11-01

Abstract: Nine isothiocyanate flavors were selected to evaluate their antibacterial activities against Staphylococcus aureus and Listeria monocytogenes by measuring the diameter of inhibition zone. Besides, the structure-activity relationship for these compounds was further investigated. The highest inhibitory effect of sulfur-containing flavors was screened to investigate its impact on the expression of the thermostable nuclease (nuc) gene of S. aureus as determined by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). The results showed that benzyl isothiocyanate (BZITC), phenethyl isothiocyanate (PEITC), 3-methylthiopropyl isothiocyanate (MTPITC) and isoamyl isothiocyanate (IAITC) had better inhibitory effect against the two Gram-positive bacteria, and the inhibitory effect followed the descending order of BZITC ≈ PEITC > MTPITC > IAITC. Among the nine isothiocyanate flavors, BZITC showed the highest inhibitory effect against S. aureus with a minimal inhibitory concentration (MIC) of 0.61 mmol/L compared to 4.88 mmol/L for L. monocytogenes. At sub-inhibitory concentrations, BZITC markedly decreased the expression of the virulence factor nuc in S. aureus (P < 0.01). These results suggested that the presence of benzene ring may enhance the antibacterial activity of isothiocyanate flavors, and play an important role in the inhibitory effect of virulence factor gene nuc expression of BZITC in S. aureus.

Key words: isothiocyanate flavors, Gram-positive bacteria, antibacterial activity, structure-activity relationship, virulence factor expression

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