Abstract: Based on the inhibition of citreoviridin (CIT) on luminol-hydrogen peroxide-nanometer copper oxide chemiluminescence system, a new method was proposed for the identification of sudden drinking water pollution. The detection system was optimized by one-factor-at-a-time and orthogonal array design methods. Results showed that under the optimized experimental conditions, the linear range for CIT determination was 0.005–3 mg/L. The limit of detection (LOD) of CIT was 8.2 × 10-5 mg/L. The precision expressed as relative standard deviation (RSD) was 2.3% for 11 replicate determinations of 0.005 mg/L CIT, and the recoveries of spiked samples were 78%–91%. This online determination method showed the advantages of convenience and strong practicability and could have a great potential application in rapid detection of sudden citreovirid pollution in drinking water.

Key words: flow injection, chemiluminescence, citreoviridin, on-line monitoring