FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (8): 163-166.doi: 10.7506/spkx1002-6630-201108037

• Processing Technology • Previous Articles     Next Articles

Preparation of High Purity Procyanidins from Raspberry Seeds by Two-step Resin Column Chromatography

ZHANG Bai-qing,ZHANG Yan-yan,LI Long-jie   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Online:2011-04-25 Published:2011-04-12

Abstract: The purpose of the present study was to develop a two-step resin column chromatographic method for purifying crude procyanidin extract from raspberry seeds. Static adsorption and desorption experiments were carried out to comparatively investigate the adsorption and desorption performance of 8 types of macroporous resins towards raspberry seed procyanidins, and type HPD100C macroporous resin showed higher adsorption quantity and desorption rate and consequently was more suitable for enriching raspberry seed procyanidins. The optimal conditions for the dynamic adsorption and desorption of raspberry seed procyanidins on type HPD100C macroporous resin were experimentally determined as follows: sample pH 5, 0.5 mL/min sample loading flow rate, and 1.5 mL/min desorption flow rate with 40% ethanol as desorption solvent. The pooled eluate obtained was applied to polyamide resin followed by desoprtion with 60% ethanol, and the resulting final purity of raspberry seed procyanidins was 92%.

Key words: raspberry seeds, procyanidins, macroporous resin, polyamide

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