FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (11): 243-247.doi: 10.7506/spkx1002-6630-201111050

• Bioengineering • Previous Articles     Next Articles

Enhancing Effect of Mutagenesis with He-Ne Laser Followed by NTG on Protease and Amylase Activities in Aspergillus oryzae

GE Jing-ping,CHEN Fang-bo,WANG Hai-man,CHAI Yang-yang,PING Wen-xiang*   

  1. Key Laboratory of Microbiology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China
  • Online:2011-06-15 Published:2011-05-13

Abstract: A protease- and amylase-producing strain named Aspergillus oryzae HDF-C14 was isolated from soybean paste and mutagenized with He-Ne laser combined with NTG. A mutant HDF-C14-HN5 was obtained and applied to the fermentation of soybean paste. The protease activity in the obtained sauce koji reached up to 1572.64 U/g and the amylase activity reached up to 2815.23 U/g. Thus, this strain can be used industrially.

Key words: Aspergillus oryzae, compound mutation, sauce koji, protease, amylase

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